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Spectral Characterization of Images
White Light Laser Confocal Microscope Leica TCS SP8 X
The white light laser source of the Leica TCS SP8 X perfectly matches
the wavelength of any fluorophore. Up to eight excitation lines can be
used – simultaneously.
By tuning both excitation and detection, complete excitation emission
spectra can be acquired.
With this spectral information, any dye can be optimally excited with
minimum cross-excitation and specimen damage.
In addition, the pulsed White Light Laser (WLL) supplies excitation
light for fluorescence lifetime measurements.
FLIM provides information about the direct environment of a molecule,
like local pH or binding to another molecule.
-Complete spectral freedom
The choice of fluorescent labels is not limited any longer by the
hardware. The researcher selects the dyes according to their
availability or the scientific needs. With up to five spectral
detection channels, the Leica TCS SP8 X is completely tunable and
fully adapts to the specimen.
-Full spectral characterization of images
New dyes or fluorescent proteins can be discovered and characterized.
A two-dimensional excitation-emission spectrum characterizes the
spectral properties of any fluorescent sample. Fluorophores with
identical emission or excitation spectra are discriminated. This is
essential for multi-color fluorescence experiments.
-Zero background by LightGate
LightGate is a clever, filter-free method to remove unwanted signal
from the image data. An adjustable time gate switches off the data
collection during the white light laser pulse. Additionally,
non-wanted fluorescence can be removed by adjusting the time gate.
This way the highest image contrast without any reflection
contribution is obtained.
-Free selection of FLIM excitation wavelength
The White Light Laser is an appropriate excitation source for
fluorescence lifetime measurements. Its continuous tunability allows
performing FLIM measurement at any excitation wavelength. Lifetime of
orange and red dyes can now be obtained. The optimal laser repetition
rate is selected via an integrated pulse picker.